Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detecting subsecond dopamine release with fast-scan cyclic voltammetry in vivo.

Donita L Robinson1, B Jill Venton, Michael L A V Heien

  • 1Department of Chemistry, Neuroscience Center, and. Center for Alcohol Studies, University of North Carolina, Chapel Hill, NC 27599-3290, USA.

Clinical Chemistry
|September 23, 2003
PubMed
Summary

Fast-scan cyclic voltammetry measures rapid dopamine fluctuations in the brain. This technique offers real-time, precise detection of dopamine signals, crucial for understanding brain function and neurological diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNAi of the electron transport chain <i>ATPsynβL</i> in glutamate neurons protects against age-related intestinal barrier dysfunction.

microPublication biology·2026
Same author

Blue light enhances background current and dopamine sensitivity of carbon-fiber microelectrodes during fast-scan cyclic voltammetry.

Analytical methods : advancing methods and applications·2026
Same author

Developmental alcohol exposure alters domains of executive function in rodents.

Physiology & behavior·2026
Same author

Reduced expression of the electron transport chain component <i>ATPsynβL</i> in glutamate neurons changes <i>Drosophila melanogaster</i> sleep patterns through adulthood.

microPublication biology·2026
Same author

Reduced central alpha power at rest is associated with the risk of alcohol-induced blackout and frequency of non-REM parasomnia episodes in adults.

Physiological reports·2026
Same author

Multiplexed neurochemical monitoring reveals glutamate modulates dopamine neurotransmission in the nucleus accumbens.

Neurochemistry international·2026

Area of Science:

  • Neuroscience
  • Analytical Chemistry
  • Electrophysiology

Background:

  • Dopamine is a key brain neuromodulator influencing motivated behaviors and neurological diseases.
  • Traditional dopamine measurements focus on slow, tonic timescales (minutes to hours).
  • Recent findings reveal dopamine fluctuates rapidly on a phasic timescale (subseconds to seconds).

Purpose of the Study:

  • To introduce and discuss fast-scan cyclic voltammetry (FSCV) for measuring brain dopamine.
  • To compare FSCV with alternative methods for dopamine detection.
  • To highlight the utility of FSCV in studying dopamine dynamics.

Main Methods:

  • Fast-scan cyclic voltammetry (FSCV) enables real-time, in vivo electrochemical analysis.
  • FSCV provides high temporal (subsecond) and spatial (micrometer) resolution.

Related Experiment Videos

  • The technique allows for selective detection of dopamine.

    • Main Results:

    • FSCV successfully measures naturally occurring dopamine transients in the rat brain.
    • Electrical stimulation of dopamine neurons yields detectable signals via FSCV.
    • Data demonstrates the capability of FSCV to capture rapid dopamine level changes.

    Conclusions:

    • Fast-scan cyclic voltammetry (FSCV) provides real-time extracellular dopamine measurements in vivo.
    • FSCV's high time resolution, spatial resolution, and chemical selectivity make it ideal for studying transient dopamine changes.
    • This technique is currently the most suitable for measuring rapid dopamine fluctuations in the brain.