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Ribozymes as tools for suppression of G protein gamma subunits
Janet D Robishaw1, Zheng-ping Guo, Qin Wang
1Weis Center for Research, Geisinger Clinic, Danville, PA, USA.
Abstract:
RNA suppression approaches provide a rapid survey of gene function. Of these approaches (i.e., antisense oligonucleotides, ribozymes, and RNA interference), ribozymes offer significant advantages by operating as stringent site-specific ribonucleases. The purpose of this chapter is to provide some guidelines and protocols for the use of ribozymes to identify the functions of closely related members of the G protein gamma subunit family. Improvements in their design and delivery will be discussed, including the use of "second generation" ribozymes targeted against multiple cleavage sites.
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