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Updated: Aug 31, 2026

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature
Published on: March 11, 2022
Crystallization and initial X-ray diffraction studies of higher plant photosystem I
Adam Ben-Shem1, Nathan Nelson, Felix Frolow
1Department of Biological Chemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel. adam@post.tau.ac.il
Abstract:
Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 A resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P2(1), with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 A, beta = 90.484 degrees. Data sets from three crystals have been collected and were merged into a 4.4 A resolution data set, taking advantage of the high isomorphicity observed for these crystals. Analysis of the self-rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit.
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