Crystallization and preliminary crystallographic analysis of RSB-66, a novel round spermatid-specific protein

Crystals of the RSB-66 protein have been grown at 291 K using NaCl as precipitant. In the refinement of the crystallization this protein, the crystallographic PCR method was used and was found to help in obtaining the best crystals more quickly and easily. The diffraction pattern of the crystal extends to 2.7 A resolution in-house. A full set of X-ray diffraction data were collected to 2.7 A from a single crystal. The crystals belong to space group P4212, with unit-cell parameters a = 90.4, b = 90.4, c = 122.2 A, alpha = beta = gamma = 90 degrees. The presence of two or three molecules per asymmetric unit gives a crystal volume per protein mass (V(M)) of 3.22 or 2.14 A(3) Da(-1), respectively.