Related Experiment Videos
Crystallization and preliminary crystallographic analysis of the importin-beta-SREBP-2 complex
Soo Jae Lee1, Toshihiro Sekimoto, Eiki Yamashita
1Institute for Protein Research, Osaka University, Japan.
Acta Crystallographica. Section D, Biological Crystallography
|September 23, 2003
Abstract:
The nuclear-transport protein importin-beta mediates the nuclear import of the transcription factor SREBP-2 without requiring adaptor proteins such as importin-alpha. An importin-beta-SREBP-2 HLHZ domain complex was purified and crystallized. The crystals belong to space group P2(1)2(1)2(1) and show diffraction to at least 3.0 A resolution. The unit-cell parameters are a = 101.0, b = 113.2, c = 240.0 A. Structure determination using the MAD or SAD method is under way.