Related Experiment Video
Updated: Aug 31, 2026

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Published on: August 20, 2018
Novel fermentation media for production of Bacillus thuringiensis subsp. israelensis
Subbiah Poopathi1, K Anup Kumar
1Centre for Research in Medical Entomology (ICMR), 4 Sarojini Street, Chinna Chokkikulam, Madurai 625002, Tamil Nadu, India.
Abstract:
The production of Bacillus thuringiensis subsp. israelensis (deBarjac) (Bti) as a biopesticide is not cost-effective using existing fermentation technology. In this study, we explored the use of several less expensive alternative culture media (potato, common sugar, and Bengal gram) for the growth and production of Bti. Growth was obtained in all tested media and was comparable to that obtained in conventional medium (Luria-Bertani). Toxicity assays showed that the toxin produced from the novel growth media were effective in killing larvae of Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti and toxicity was comparable to that produced from Luria-Bertani medium. These observations suggest that potato can be used as a cheap source of culture medium for the production of Bti toxin in mosquito control programs.
Related Concept Videos
Production of Biopesticides
Microbes in the Production of Fermented Foods
Microbes in Food Production
Bioreactor Controls-III
Production of Organic Acids
Microbial Growth Media

