Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Macroenzyme detection by polyethylene glycol precipitation.

D Fraser Davidson1, Dawn J M Watson

  • 1Biochemistry Department, Crosshouse Hospital, Kilmarnock KA2 0BE, UK. fraser.davidson@aaaht.scot.nhs.uk

Annals of Clinical Biochemistry
|September 25, 2003
PubMed
Summary

This study introduces a simple polyethylene glycol precipitation method for detecting macroenzymes, which can cause diagnostic confusion. The new technique offers an effective way to identify these large enzyme variants in plasma.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

L-DOPA therapy interferes with urine catecholamine analysis in children with suspected neuroblastoma: a case series.

Annals of clinical biochemistry·2016
Same author

A survey of some pre-analytical errors identified from the Biochemistry Department of a Scottish hospital.

Scottish medical journal·2014
Same author

Urinary free (unconjugated) metadrenalines in different hereditary forms of catecholamine-secreting phaeochromocytoma/paraganglioma.

Annals of clinical biochemistry·2012
Same author

Detection of creatine kinase macroenzymes.

Annals of clinical biochemistry·2012
Same author

A phaeochromocytoma occurring in a patient with Parkinson's disease on L-dopa therapy: a diagnostic challenge.

Clinical endocrinology·2011
Same author

Age-related medical decision limits for urinary free (unconjugated) metadrenalines, catecholamines and metabolites in random urine specimens from children.

Annals of clinical biochemistry·2011

Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Enzymology

Background:

  • Macroenzymes, or large enzyme variants, present diagnostic challenges.
  • Current detection methods for macroenzymes can be complex and time-consuming.

Observation:

  • A simplified polyethylene glycol (PEG) precipitation technique combined with isoenzyme electrophoresis was developed.
  • Reference ranges for precipitable activity were established for seven common plasma enzymes.

Findings:

  • The study established reference ranges for macroenzyme detection using PEG precipitation.
  • Twelve cases of macroenzymaemia were identified using the new protocol.
  • Three representative case histories illustrating macroenzyme detection are detailed.

Related Experiment Videos

Implications:

  • The PEG precipitation method provides a straightforward and efficient tool for identifying macroenzymes.
  • This technique aids in resolving diagnostic confusion arising from elevated plasma enzyme activity.
  • The findings support the clinical utility of PEG precipitation in routine diagnostics.