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Microtubule organization in lymphoid malignancies
1Department of Internal Medicine, Kanazawa Medical University, Ishikawa Pref., Japan.
Blood Cells
|January 1, 1992
Summary
Microtubules in neoplastic lymphocytes from T and B cell lymphoid malignancies are generally well-organized. Stimuli like PHA-P and Con A promote microtubule development, correlating with cell proliferation in these cancers.
Area of Science:
- Cell Biology
- Immunology
- Oncology
Background:
- Abnormal actin and vimentin organization is known in neoplastic lymphocytes.
- Microtubule organization in these cells requires further investigation.
Purpose of the Study:
- To investigate the organization and behavior of microtubules in T and B cell lymphoid malignancies.
- To assess the impact of blastogenic stimuli and specific factors on microtubule structure.
Main Methods:
- Immunofluorescence microscopy using monoclonal tubulin antibody.
- Analysis of cells from 34 lymphoid malignancy cases.
- In vitro culture with mitogens (PHA-P, Con A, PWM) and cytokines (interferon alpha, G-CSF).
Main Results:
- Most leukemia, lymphoma, and myeloma cells exhibited well-organized microtubules.
- Microtubules became visible in cultured T cell lymphoma cells after stimulation.
- Irregularly shaped nuclei in certain leukemia/lymphoma cells correlated with complex microtubule arrangements.
- Microtubule development was proportional to 3H-thymidine incorporation after blastogenic stimuli.
- G-CSF and interferon alpha increased cell degeneration but intact cells retained microtubules.
Conclusions:
- Microtubules are generally well-organized in T and B cell lymphoid malignancies.
- Microtubule organization can be influenced by cellular morphology and external stimuli.
- Further research into microtubule dynamics may offer insights into lymphoid malignancy progression.