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[Skin tissue engineering].

H Bannasch1, M Föhn, T Unterberg

  • 1Abteilung Plastische und Handchirurgie, Chirurgische Universitätsklinik Freiburg. bannasch@ch11.ukl.uni-freiburg.de

Der Chirurg; Zeitschrift Fur Alle Gebiete Der Operativen Medizen
|September 25, 2003
PubMed
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Cultured keratinocytes offer a promising alternative for skin regeneration, showing reliable wound reepithelialization in 14 days. Combining keratinocyte suspension with decellularized dermis improves outcomes for full-thickness skin defects.

Area of Science:

  • Tissue Engineering
  • Regenerative Medicine
  • Dermatology

Background:

  • Cultivated epithelial autografts (CEAs) are standard tissue engineering substitutes for skin.
  • CEAs often yield suboptimal mechanical stability and scarring.
  • Alternatives to CEAs are needed for improved skin regeneration.

Purpose of the Study:

  • To investigate cultured autologous keratinocytes in a fibrin matrix as an alternative to CEAs.
  • To evaluate the efficacy of keratinocyte-fibrin suspension combined with acellular dermis for full-thickness skin defects.

Main Methods:

  • Cultured autologous keratinocytes were used as a single cell suspension in a fibrin matrix.
  • Transplantation studies were conducted in athymic nude mice and a pig model.
  • Keratinocyte suspension was combined with acellular dermis for full-thickness defect regeneration.

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Main Results:

  • Reliable wound reepithelialization was achieved in 14 days using cultured keratinocytes.
  • Successful regeneration of full-thickness skin defects was demonstrated in pigs using keratinocyte fibrin suspension and acellular dermis.
  • Subconfluently cultured keratinocytes, harvested early and easily handled, showed enhanced utility when cotransplanted with decellularized dermis.

Conclusions:

  • Cultured keratinocytes in fibrin matrix represent a viable alternative for skin tissue engineering.
  • Cotransplantation with decellularized dermis enhances the regenerative potential of keratinocytes for full-thickness skin defects.
  • This approach offers improved outcomes compared to traditional cultivated epithelial autografts.