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Facilitated forward chemical genetics using a tagged triazine library and zebrafish embryo screening
Sonya M Khersonsky1, Da-Woon Jung, Tae-Wook Kang
1Department of Chemistry, New York University, New York, New York 10003, USA.
Journal of the American Chemical Society
|September 25, 2003
Abstract:
An improved forward chemical genetics approach was successfully demonstrated using a tagged library concept. A small-molecule triazine library with linkers was used to screen for brain/eye developmental phenotypes in a zebrafish embryo system. This approach enabled the rapid isolation of the target proteins by facile affinity matrix preparation and elucidated the first small-molecule inhibitors for several ribosomal accessory proteins or their complex as the target.

