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Published on: February 24, 2026
Hospital use of decontaminating mats
M G Marchetti1, G Finzi, P Cugini
1University of Ferrara, Italy.
Abstract:
Decontaminating mats made of several layers of adhesive sheets (water-based acrylic 6 g/m2) supplemented with a bactericidal agent (3-1 benzoisothiazolin) at a concentration of 25% were placed in the passages providing access to the operating rooms of an orthopaedic service. Contact plates containing tryptone soy agar were used to assess bacterial concentration at specific points in front of and beyond the mats. For trolley passageways two areas were defined: central and lateral paths, corresponding to the areas walked upon by the personnel pushing the trolleys and to the paths covered by the trolley wheels, respectively. In order to exclude a simple mechanical effect, a comparison of bacterial loads at defined sites beyond the mats was carried out in the presence and in the absence of decontaminating mats. Bacterial colony counts in the presence of decontaminating mats were substantially and statistically significantly reduced compared with the absence of mats. The lower mean number of colony-forming units detected at points located beyond the mats parallels this finding; this difference is also statistically significant. We thus conclude that decontaminating mats are potentially useful in decreasing micro-organism carry-over due to personnel or the passage of trolleys into areas at high risk of infection such as operating rooms.
Insights
Bactericidal decontaminating mats significantly reduced bacterial counts in operating room access areas. These mats effectively decrease microorganism transfer from personnel and trolleys, enhancing operating room hygiene.
Area of Science:
- Healthcare-associated infection control
- Microbiology
- Environmental hygiene
Background:
- Operating rooms require stringent infection control measures.
- Preventing microorganism transfer into sterile environments is crucial.
- Personnel and equipment movement can introduce contaminants.
Purpose of the Study:
- To evaluate the efficacy of bactericidal decontaminating mats in reducing bacterial load.
- To assess the impact of mats on microorganism transfer into orthopaedic operating rooms.
Main Methods:
- Adhesive decontaminating mats with a bactericidal agent were installed.
- Contact plates with tryptone soy agar assessed bacterial concentration.
- Bacterial loads were compared with and without mats at various locations.
Main Results:
- Bacterial colony counts were substantially and significantly reduced with mats.
- Fewer colony-forming units were detected beyond the mats, indicating reduced transfer.
- Both personnel and trolley pathways showed significant bacterial reduction.
Conclusions:
- Decontaminating mats are effective in reducing bacterial contamination in operating room access points.
- These mats represent a valuable tool for infection prevention in high-risk areas.
- Implementation can decrease the risk of microorganism carry-over into operating rooms.
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