Bis-pyrene labeled DNA aptamer as an intelligent fluorescent biosensor

Area of Science:

• Biochemistry
• Molecular Biology
• Analytical Chemistry

Background:

• DNA aptamers are versatile molecules for molecular recognition.
• Fluorescent sensors offer sensitive detection methods.
• Adenosine triphosphate (ATP) is a crucial biological analyte.

Purpose of the Study:

• To develop a novel, highly sensitive fluorescent sensor for adenosine triphosphate (ATP).
• To investigate the utility of site-directed fluorophore incorporation in DNA aptamers.

Main Methods:

• Site-directed incorporation of a bis-pyrenyl fluorophore into an anti-ATP DNA aptamer sequence.
• Characterization of the resulting aptamer-fluorophore conjugate.
• Testing the sensor's performance in a homogeneous detection system for ATP.

Main Results:

• The modified DNA aptamer exhibited high fluorescence signal intensity upon binding to ATP.
• The sensor demonstrated excellent specificity for ATP detection over other related molecules.
• Homogeneous detection of ATP was achieved with high sensitivity.

Conclusions:

• Site-directed fluorophore incorporation is an effective strategy for creating intelligent aptasensors.
• The developed bis-pyrenyl-functionalized anti-ATP aptamer sensor is a promising tool for sensitive and specific ATP detection.