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Related Experiment Videos

Rapid anionic micelle-mediated alpha-synuclein fibrillization in vitro.

Mihaela Necula1, Carmen N Chirita, Jeff Kuret

  • 1Biophysics Program, The Ohio State University College of Medicine and Public Health, Columbus, OH 43210, USA.

The Journal of Biological Chemistry
|September 25, 2003
PubMed
Summary

Fatty acids and anionic detergents accelerate alpha-synuclein fibrillization, a key process in Parkinson's disease. Anionic surfaces, like micelles and vesicles, act as nucleation sites for this aggregation.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Parkinson's disease involves alpha-synuclein aggregation in neurons.
  • In vitro fibrillization of alpha-synuclein is typically inefficient.
  • Previous studies showed enhancement by glycosaminoglycans and polycations.

Purpose of the Study:

  • To investigate the effect of fatty acids and anionic detergents on alpha-synuclein fibrillization.
  • To determine the mechanism by which these agents accelerate aggregation.
  • To explore the role of anionic surfaces in alpha-synuclein nucleation.

Main Methods:

  • Recombinant alpha-synuclein fibrillization assays.
  • Use of fatty acids, anionic detergents, and anionic phospholipid vesicles.

Related Experiment Videos

  • Measurement of lag times and growth rate constants.
  • Critical micelle concentration determination.

    • Main Results:

    • Fatty acids and anionic detergents significantly accelerated alpha-synuclein fibrillization at low micromolar concentrations.
    • Observed lag times as short as 11 min and rate constants up to 10.4 h-1.
    • Detergents and fatty acids formed micelles that enhanced aggregation.
    • Anionic phospholipid vesicles also induced fibrillization, with filaments originating from the vesicle surface.

    Conclusions:

    • Anionic surfaces, including micelles and vesicles, can nucleate alpha-synuclein fibrillization.
    • This nucleation mechanism explains the activity of anionic surfactants in promoting aggregation.
    • Anionic membranes may play a role in in vivo alpha-synuclein fibrillization.