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Updated: Aug 31, 2026
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
Separation and identification of exo- and endoinulinase from Aspergillus ficuum
Wang Jing1, Jin Zhengyu, Jiang Bo
1School of Food Science and Technology, Southern Yangtze University, 170 Huihe Road, Wuxi-214036, JiangSu Province, P. R. China.
Abstract:
A new and convenient method was developed to separate and identify exo- and endoinulinase from Aspergillus ficuum by native polyacrylamide gel electrophoresis. Eight protein bands were obtained. Three bands were identified as exoinulinase, and two bands were endoinulinase, by using TLC and HPLC. The five bands were all detected as glycoproteins with the sensitive periodic acid-silver stain.
