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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
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Direct detection and quantification of horizontal gene transfer by using flow cytometry and gfp as a reporter gene

Søren J Sørensen1, Anders H Sørensen, Lars H Hansen

  • 1Department of General Microbiology, Institute of Molecular Biology, University of Copenhagen, Sølvgade 83 H, DK 1307, Copenhagen, Denmark. SJS@MERMAID.MOLBIO.KU.DK

Current Microbiology
|September 26, 2003
PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
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Horizontal Gene Transfer

Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...