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Selective binding of TAR RNA by a Tat-derived beta-peptide
Michael A Gelman1, Sara Richter, Hong Cao
1Department of Chemistry and Medical Scientist Training Program, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA. gellman@chem.wisc.edu
Organic Letters
|September 26, 2003
Abstract:
[structure: see text] The interaction between the HIV-1 Tat protein and the TAR RNA element in the nascent viral genomic transcript is required for viral replication. An 11-residue beta-peptide (1), an all-beta homologue of the Arg-rich region Tat 47-57, binds TAR RNA with K(d) = 29 +/- 4 nM. A control beta-peptide (2) in which all Arg side chains are replaced by Lys side chains shows increased affinity but decreased specificity for wild-type vs bulge-deleted TAR RNA, as do the alpha-peptide analogues of 1 and 2.