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Related Experiment Videos

Vascular endothelial growth factor modulates skeletal myoblast function.

Antonia Germani1, Anna Di Carlo, Antonella Mangoni

  • 1Laboratorio di Biologia Vascolare e Terapia Genica, Centro Cardiologico Fondazione I. Monzino, Istituto di Ricovero e Cura a Carattere Scientifico, Milano, Italy. a.germani@idi.it

The American Journal of Pathology
|September 26, 2003
PubMed
Summary

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Vascular endothelial growth factor (VEGF) promotes skeletal muscle repair by enhancing myoblast migration and survival during ischemia. This study reveals VEGF

Area of Science:

  • Muscle regeneration and repair
  • Molecular biology
  • Angiogenesis

Background:

  • Vascular endothelial growth factor (VEGF) is crucial for angiogenesis in ischemic tissues.
  • Its role in modulating skeletal muscle cell function beyond blood vessel growth is unclear.

Purpose of the Study:

  • To investigate the function of VEGF in skeletal muscle cells, particularly satellite cells and myoblasts, during ischemic conditions.
  • To determine if VEGF influences myoblast migration, survival, and differentiation.

Main Methods:

  • Immunohistochemical analysis of VEGF and its receptors (Flk-1, Flt-1) in mouse hindlimb muscle.
  • In vitro studies using cultured satellite cells and C2C12 myoblasts.
  • VEGF treatment, receptor inhibition, and adenovirus-mediated gene transfer experiments.

Related Experiment Videos

  • Cell migration assays and apoptosis assessments.

    • Main Results:

    • VEGF and its receptors are expressed in quiescent satellite cells and upregulated in regenerating muscle fibers post-ischemia.
    • VEGF enhances myoblast migration and prevents apoptosis in differentiating myoblasts.
    • Inhibition of VEGF signaling increases myoblast cell death.
    • VEGF gene transfer reduces ischemia-induced apoptosis in skeletal muscle.

    Conclusions:

    • VEGF plays a significant role in myoblast migration and survival, contributing to skeletal muscle repair.
    • A novel autocrine function of VEGF in skeletal muscle regeneration during ischemia is suggested.