Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Site-specific dichroism analysis utilizing transmission FTIR.

Eyal Arbely1, Itamar Kass, Isaiah T Arkin

  • 1The Alexander Silberman Institute of Life Sciences, Department of Biological Chemistry, The Hebrew University, Givat-Ram, Jerusalem, Israel.

Biophysical Journal
|September 26, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine-learning prediction of affinity and epistasis in the bovine pancreatic trypsin inhibitor-chymotrypsin complex.

Protein science : a publication of the Protein Society·2026
Same author

Prostate cancer risk-associated single-nucleotide polymorphisms impact the conformational dynamics of prostate-specific antigen.

BMC biology·2025
Same author

Isoform-specific regulation of PKM by acetylation.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Ion-Channel-Targeting Drugs for Chikungunya Virus.

Molecules (Basel, Switzerland)·2025
Same author

Identification and Structural Characterization of Viroporins from Deadly Hemorrhagic Viruses.

Viruses·2025
Same author

A bacteria-based search for drugs against avian and swine flu yields a potent and resistance-resilient channel blocker.

Proceedings of the National Academy of Sciences of the United States of America·2025

Site-specific infrared dichroism (SSID) now uses transmission spectroscopy for protein structural analysis. This method provides accurate, localized orientational restraints for amino acids, improving membrane protein structure determination.

Area of Science:

  • Biophysics
  • Structural Biology
  • Spectroscopy

Background:

  • Infrared spectroscopy traditionally analyzes average protein secondary structure and orientation.
  • Site-specific isotope labeling enables examination of localized protein properties.

Purpose of the Study:

  • To extend site-specific infrared dichroism (SSID) using transmission infrared spectroscopy.
  • To develop theory and demonstrate accurate site-specific orientational restraints from transmission experiments.

Main Methods:

  • Utilized site-specific isotope labeling (e.g., peptidic 1-(13)C = (18)O).
  • Applied transmission infrared spectroscopy tilt series.
  • Developed corresponding theoretical framework.

Main Results:

Related Experiment Videos

  • Demonstrated accurate site-specific orientational restraints can be derived.
  • Extended SSID capabilities beyond attenuated total internal reflection.
  • Enabled site-specific analysis of localized protein properties.

Conclusions:

  • Transmission infrared spectroscopy is a viable method for site-specific orientational analysis.
  • This approach enhances the determination of membrane protein backbone structures.
  • SSID using transmission offers a powerful tool for structural biology.