Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A decrease in the expression of CD63 tetraspanin protein elevates invasive potential of human melanoma cells.

Hwa-In Jang1, Hansoo Lee

  • 1Vascular System Research Center, Division of Life Sciences, Kangwon National University, Chunchon 200-701, Korea.

Experimental & Molecular Medicine
|September 26, 2003
PubMed
Summary

Reduced CD63 protein expression in melanoma cells promotes metastasis. Lowering CD63 levels increased cell motility and invasiveness, suggesting CD63 inhibits melanoma spread.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Waterfowl Move Less in Heterogeneous and Human-Populated Landscapes, With Implications for Spread of Avian Influenza Viruses.

Ecology letters·2026
Same author

Causal interactions among phytoplankton and Pseudo-nitzschia species revealed by empirical dynamic modelling.

Marine pollution bulletin·2024
Same author

Flyways and migratory behaviour of the Vega gull (Larus vegae), a little-known Arctic endemic.

PloS one·2023
Same author

Macrophage inhibitory cytokine-1 produced by melanoma cells contributes to melanoma tumor growth and metastasis in vivo by enhancing tumor vascularization.

Melanoma research·2021
Same author

Incorporation of Celiomesenteric Trunk With Double Kissing Directional Branches During Fenestrated-Branched Endovascular Aortic Repair.

Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists·2021
Same author

Ecological insights from three decades of animal movement tracking across a changing Arctic.

Science (New York, N.Y.)·2020

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • CD63 is a tetraspanin membrane protein implicated in inhibiting melanoma metastasis.
  • Reduced CD63 expression is observed during the malignant progression of human melanoma.

Purpose of the Study:

  • To investigate if decreased CD63 expression promotes melanoma metastasis.
  • To determine the functional role of CD63 in melanoma cell invasiveness and motility.

Main Methods:

  • Transfection of antisense CD63 cDNA into MelJuso melanoma cells to reduce CD63 expression.
  • In vitro assessment of cell motility, matrix-degrading activity, and invasiveness in CD63-reduced cells compared to controls.
  • Analysis of altered adhesiveness to extracellular matrix in transfected cells.

Related Experiment Videos

Main Results:

  • Transfectant clones with decreased CD63 expression showed significantly increased cell motility.
  • Matrix-degrading activity and invasiveness were elevated in cells with reduced CD63 expression.
  • Altered adhesiveness to extracellular matrix was observed in antisense CD63 cDNA-transfected cells.

Conclusions:

  • Reduced CD63 expression is a contributing factor to the invasive and metastatic potential of human melanoma cells.
  • CD63 plays a crucial role in suppressing melanoma cell invasion and metastasis.