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Related Experiment Videos

Enzyme mass-transfer coefficient in aqueous two-phase systems using static mixer extraction column.

K Rostami1, M Alamshahi

  • 1No. 71 Forsat Street, Biotechnology Center, Iranian Research Organization for Science and Technology, Tehran-15819, Iran. rostami2002@yahoo.com

Bioprocess and Biosystems Engineering
|September 26, 2003
PubMed
Summary

This study optimized alpha-amylase extraction using aqueous two-phase systems (ATPS) in a spray column. Higher dispersed phase velocity improved extraction efficiency, with correlations developed for key parameters.

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Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Process Intensification

Background:

  • Aqueous two-phase systems (ATPS) are advanced for biomacromolecule extraction.
  • Alpha-amylase purification is crucial in various biotechnological applications.
  • Spray columns offer potential for continuous ATPS operations.

Purpose of the Study:

  • To investigate the extraction of alpha-amylase using sodium sulphate-polyethylene glycol (PEG) ATPS.
  • To analyze the impact of operational parameters on extraction efficiency in a spray column.
  • To develop empirical correlations for hold-up and mass transfer coefficients.

Main Methods:

  • Utilized sodium sulphate-polyethylene glycol (PEG) in water to form ATPS.
  • Employed a 47-mm inner diameter spray column with static mixers.

Related Experiment Videos

  • Varied dispersed-phase flow rate, phase composition, column height, and diameter.
  • Operated the system in a semi-batch mode.

    • Main Results:

    • Hold-up and volumetric mass transfer coefficients increased with dispersed (PEG-rich) phase velocity.
    • Higher phase composition led to decreased hold-up and mass transfer coefficients.
    • Empirical correlations were established for fractional dispersed-phase hold-up and volumetric mass transfer coefficients.

    Conclusions:

    • Optimized ATPS conditions in a spray column enhance alpha-amylase extraction.
    • Understanding parameter effects is key for efficient biomacromolecule separation.
    • Developed correlations can aid in the design and scale-up of ATPS processes.