Related Experiment Video
Updated: Aug 31, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Electrochemical detection of single-base mismatches in DNA by a redox-active intercalator conjugated oligonucleotide
Kazushige Yamana1, Naoko Kawakami, Takayoshi Ohtsuka
1Department of Applied Chemistry, Himeji Institute of Technology, 2167 Shosha, Himeji 671-2201, Japan.
Abstract:
A simple and direct electrochemical detection of DNA single-base mismatches utilizing anthraquinone-modified oligonucleotides (AQ-ODNs) bound to gold electrodes has been described. By using the redox-active oligonucleotides, the redox-active center can be positioned at the desired base-pair pocket in double-helical DNA, enabling us to measure the charge transport between the redox-active center and the electrode that sandwich mismatched and full-matched base-pairs in double helix. AQ-ODNs immobilized on gold electrodes have been found to be useful in the electrochemical discrimination of hybrids containing a single-base mismatch from the one with fully matched bases.
Related Concept Videos
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Mismatch Repair
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Base Excision Repair
The first step of...
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

