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Updated: Jul 19, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 12, 2010
Identification of functional genes by libraries of ribozymes and siRNAs
Hiroaki Kawasaki1, Tomoko Kuwabara, Makoto Miyagishi
1Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Abstract:
Libraries were made of hammerhead ribozymes with randomized binding arms and/or of U6 and tRNA-driven siRNAs. Then, the libraries were introduced into cells either by transfection or by viral vectors. This procedure made it possible to readily identify the relevant genes associated with phenotype in the apoptosis, cancer metastasis, and/or cell differentiation pathways. This application of a randomized library represents a simple and yet powerful method for identification of functional genes associated with specific phenotypes in the post-genome era. Importantly, the identified functional genes originated from not only the coding region but also the noncoding region.
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