Related Experiment Videos
Technical issues in construction of nucleic acid vaccines
Peter F Ertl1, Lindy L Thomsen
1Department of Immunotherapeutics, GlaxoSmithKline, Gunnels Wood Road, Stevenage, SG1 2NY Hertfordshire, UK.
Methods (San Diego, Calif.)
|September 27, 2003
Abstract:
Delivery of antigen-encoding genes by nucleic acid vaccine vectors offers tremendous advantages in power and flexibility over conventional antigen delivery systems. Of the many forms of nucleic acid vaccine that can be constructed, circular DNA plasmids are the simplest. In this article, we consider the components that make up a generic DNA plasmid vaccine. We discuss some of the issues encountered when optimizing these vectors to exploit their potential for in vivo expression and presentation of antigens and thereby maximizing the immune response.