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An immuno-affinity method for the purification of mannose 6-phosphate receptor proteins
Koduru Suresh1, Siva Kumar Nadimpalli
1Protein Biochemistry Laboratory, Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, India.
Journal of Biochemical and Biophysical Methods
|September 27, 2003
Abstract:
In a recent study, we have developed an ELISA method to quantify the mannose 6-phosphate receptor (MPR) proteins [J. Biochem. Biophys. Methods 52 (2002) 111]. In the present study, we have used the goat MPR 300 antibody and peptide specific antibodies to human MPR 46 to develop simple and efficient immuno-affinity matrices, which can be used to purify the MPR proteins from goat liver in a single step. The identity of the immuno-affinity purified receptors is confirmed by their molecular masses as well as by their immunoreactivity.