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Related Experiment Videos

Intracellular pH in hypoxic smooth muscle.

N L Stephens, E A Kroeger, W Loh

    The American Journal of Physiology
    |March 1, 1977
    PubMed
    Summary

    Hypoxia increases lactate in canine tracheal smooth muscle (TSM), indicating increased glycolysis. Unexpectedly, muscle activity during hypoxia raises intracellular pH, contradicting the expected lactacidosis effect.

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    Area of Science:

    • Physiology
    • Biochemistry

    Background:

    • Hypoxia is known to impair contractility in canine tracheal smooth muscle (TSM).
    • This impairment has been attributed to intracellular lactacidosis.

    Purpose of the Study:

    • To investigate the relationship between hypoxia, lactate production, and intracellular pH (pHi) in TSM.
    • To determine if intracellular lactacidosis occurs under hypoxic conditions in TSM.

    Main Methods:

    • Measurement of lactate levels in hypoxic and normoxic TSM.
    • Measurement of intracellular pH (pHi) in resting and active TSM under both normoxic and hypoxic conditions.

    Main Results:

    • Lactate levels were significantly higher in hypoxic TSM compared to normoxic TSM.
    • Active TSM under hypoxia showed a significant increase in pHi, contrary to the expected decrease.
    • Resting TSM under hypoxia did not show a significant change in pHi compared to resting normoxic TSM.

    Conclusions:

    • Hypoxia induces increased glycolysis in TSM, evidenced by elevated lactate production.
    • Mechanical activity in hypoxic TSM leads to a paradoxical rise in intracellular pH.
    • The expected intracellular lactacidosis does not occur in active hypoxic TSM; instead, pHi increases.

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