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Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
Cytokine production and serum levels in systemic sclerosis
T V Kantor1, D Friberg, T A Medsger
1Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania.
Clinical Immunology and Immunopathology
|December 11, 1992
Summary
Systemic sclerosis patients exhibit elevated spontaneous production of tumor necrosis factor-alpha and interleukin 1-beta by peripheral blood mononuclear cells. These findings suggest these cytokines contribute to connective tissue changes in systemic sclerosis.
Area of Science:
- Immunology
- Rheumatology
- Cell Biology
Background:
- Systemic sclerosis (SSc) is a complex autoimmune disease characterized by fibrosis.
- Cytokine dysregulation is implicated in the pathogenesis of SSc.
Purpose of the Study:
- To investigate serum cytokine levels and peripheral blood mononuclear cell (PBMNC) production in SSc patients.
- To correlate cytokine profiles with disease subsets.
Main Methods:
- Measurement of serum cytokines (TNF-alpha, IL1-beta, IL2, sIL2R) and PBMNC production (TNF-alpha, IL1-beta, IFN-gamma) via immunoassays.
- Patient stratification into limited cutaneous SSc (lcSSc) and diffuse cutaneous SSc (dcSSc) based on duration.
Main Results:
- Elevated serum soluble IL2 receptors (sIL2R) in SSc patients.
- Significantly higher spontaneous production of TNF-alpha and IL1-beta by PBMNC from SSc patients compared to controls.
- Early dcSSc patients showed the greatest increase in spontaneous TNF-alpha and IL1-beta release.
- Mitogen-induced IFN-gamma production was significantly depressed in SSc patients.
Conclusions:
- In vivo-activated PBMNC in SSc patients spontaneously secrete excessive amounts of fibrogenic cytokines.
- These cytokines may play a crucial role in modulating connective tissue synthesis in SSc.
- Cytokine profiles differ across SSc disease subsets, particularly in early dcSSc.
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