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Updated: May 24, 2026
Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood
Published on: April 13, 2012
[Cloning and sequencing of human vascular endothelial growth factor-C encoded cDNA]
Jian Pan1, Chengge Hua, Yuming Wen
1Department of Oral Maxillofacial Surgry, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Objective:
A functional VEGF-C cDNA was cloned from a patient with squamous cell carcinoma (SCC) of tongue in order to study the important role of vascular endothelial growth factor (VEGF)-C in lymphatic dissemination of malignancies in gene level.
Methods:
RT-PCR was employed to clone the human VECF-C encoded cDNA from a surgical specimen of a lingual SCC patient. Then it was subcloned into plasmid vector pCRII and sequenced.
Results:
A 1.1 kb human VEGF-C cDNA fragment was amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99.6% similarity with the reported human VEGF-C cDNA (representing the 559-1,611 bp according the sequence of Genbank Entry X94216).
Conclusion:
An encoded fragment VEGF-C cDNA was successfully cloned from a lingual SCC and provided a necessary material for further study.
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