[Prokaryotic expression of vascular endothelial growth factor-C]

To evaluate whether the vascular endothelial growth factor (VEGF)-C cDNA which cloned from a patient with squamous cell carcinoma (SCC) of tongue can encode a functional protein or not.

RT-PCR was employed to clone the functional VEGF-C fragment from the surgical specimen of a lingual SCC patient. Then it was subcloned into expressive plasmid vector pBKCMV, which was transfected into E. coli to examine its expression.

A truncated human VEGF-C cDNA fragment was amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99.6% similarity with the reported human VEGF-C cDNA (representing the 559-1611 bp according the sequence of Genbank Entry X94216). Induced with IPTG, the E. coli XLI-Blue MRF' containing the recombinant pBK-VEGF-C expressed a 56,000 fusion protein, which can be recognized by polyclonic anti-human VEGF-C antibody.

A functional fragment VEGF-C cDNA was cloned from a lingual SCC. It will promote more intensive research on the function of VEGF-C and its relationship with metastasis of oral SCC.