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Updated: Aug 29, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Effect of immunological orchitis on sperm specific enzyme and fertility]
Lei Chen1, Wei-Ping Xia, Zhi-Heng Zhou
1Department of Urology, Longhua Hospital, Shanghai University of Traditiond Chinese Medicine, Shanghai 200032, China.
Objective:
To investigate the effect of immunological orchitis on spermatic specific enzyme and fertility.
Methods:
Experimental allergic orchitis (EAO) model of guinea pigs was duplicated. The histological and morphological changes of spermatic acrosomal protease and hyaluronidase, lactate dehydrogenase, sperm in epididymis and testes were observed by means of enzyme kinetical spectrophotometry and gelatin fixation of substrate thin membrane.
Results:
The activity of acrosomal protease, hyaluronidase and spermatic cytoplasmic lactic dehydrogenase in the epididymis acrosomal enzyme system became low, and so did the quality of sperm in epididymis. Remarkable morphological changes of spermatogenic cells developed in the convoluted seminiferous tubules.
Conclusions:
EAO remarkably affects the fertility of male guinea pigs. The orchis and epididymal sperms might be the sites of action.
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