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Published on: July 30, 2011
[Molecular cloning and identification of calmegin gene in embryo and adult testes by cDNA microarray hybridization]
Ting Cui1, Hui Zhu, Li-Rong Wang
1Department of Clinical Laboratory, First Affiliated Hopital, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Objective:
To identify gene differential expression in embryo and adult testes by cDNA microarray techniques.
Methods:
cDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed.
Results:
The calmegin (CLGN) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20-fold difference at expression level between adult and embryo human testes.
Conclusion:
Genes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization.

