Measurement of whole blood phagocyte chemiluminescence in a microtitreplate format

Zymosan-induced luminol-enhanced chemiluminescence of phagocytes was measured in whole blood using a microtitreplate format. A consistent reaction response curve could be obtained using a normal blood sample, with low intra-assay variations in peak light index, cumulated response as well as time taken to reach the peak. Variations were common when tests were done on different days for the same individual, or when different persons were compared simultaneously. Reactions were hastened and enhanced if continuous shaking at 37 degrees C was applied. The interval between time zero and peak was the most consistent variable, with a coefficient of variation of 12% in inter-assay analysis. Because of its simplicity and ease of operation, the method is potentially useful in studying phagocyte functions in pathological states.