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Updated: Aug 30, 2026
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Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
Published on: January 17, 2025
Methods to quantify the biotin-binding capacity of streptavidin-coated polypropylene PCR plates
Anne M van der Valk1, David N Howbrook, Meg C O'Shaughnessy
1ABgene, ABgene House, Blenheim Road, Epsom, Surrey KT19 9AP, UK.
Biotechnology Letters
|September 30, 2003
Abstract:
The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respectively.
