Progesterone stimulates prostaglandin synthesis in eggshell gland mucosa of estrogen-primed chickens

1. Prostaglandins may be involved in calcium translocation in the avian shell gland, since indomethacin, administered at the beginning of shell formation, reduces eggshell thickness as well as 45Ca-uptake and prostaglandin synthesis by a homogenate of eggshell gland mucosa. 2. The stimulus for calcium transport in the shell gland during shell formation remains unknown. 3. The present study was undertaken to investigate the effects of progesterone on prostaglandin formation by the eggshell gland mucosa of the domestic fowl. 4. Progesterone significantly stimulated synthesis of PGF2 alpha, PGE2 and TXB2 by eggshell gland mucosa homogenate. 5. Progesterone treatment also induced the synthesis of the biotin-binding protein, avidin. 6. A microsomal fraction prepared from the eggshell gland mucosa had a high affinity for binding PGE2. 7. Progesterone treatment reduced the KD value of this binding without affecting the maximal number of binding sites. 8. Progesterone did not change the total calcium content of shell gland mucosa. 9. The role progesterone plays in prostaglandin formation and calcium transport in the eggshell gland mucosa is discussed.