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Updated: Aug 30, 2026
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
Published on: March 10, 2017
Complex analysis of genes involved in the inflammatory response: interleukin-1-induced differential transcriptome of
1Department of Cellular Biochemistry, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland. koj@mol.uj.edu.pl
Abstract:
The systemic inflammatory reaction (acute phase response) is induced by many noxious stimuli but in all cases the inflammatory cytokines, such as interleukin-1-beta (IL-1beta) and interleukin-6 (IL-6), are involved. Liver cell response to inflammation manifested by a characteristic change in the profile of synthesized plasma proteins (acute phase proteins) has been extensively studied. Here we describe a model system of cultured human hepatoma HepG2 cells stimulated with IL-1beta to evaluate the transcriptome induced by this cytokine during 24 h of treatment. By using differential display analysis we found IL-1beta-induced upregulation of several genes coding for cellular trafficking/motor proteins, proteins participating in the translation machinery or involved in posttranscription/posttranslation modifications, proteases, proteins involved in cellular metabolism, activity modulators, proteins of the cell cycle machinery and also some new proteins so far functionally not classified.
