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Related Experiment Videos

Circulating endothelial cells in pulmonary hypertension.

Todd M Bull1, Heiko Golpon, Robert P Hebbel

  • 1Division of Pulmonary Sciences and Critical Care Medicine, Pulmonary Hypertension Center, University of Colorado School of Medicine, Denver, CO 80262, USA. Todd.Bull@UCHSC.edu

Thrombosis and Haemostasis
|September 30, 2003
PubMed
Summary

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Patients with pulmonary hypertension have significantly more circulating endothelial cells (CECs) in their blood. CEC numbers correlate with pulmonary artery pressure, suggesting they may indicate disease severity.

Area of Science:

  • Cardiovascular Biology
  • Pulmonary Medicine
  • Endothelial Cell Biology

Background:

  • The pulmonary endothelium is crucial in the pathology of Primary Pulmonary Hypertension (PPH).
  • Vascular injury is linked to increased circulating endothelial cells (CECs).
  • Pulmonary hypertension involves high pressures and shear stress, potentially increasing CECs.

Purpose of the Study:

  • To investigate if patients with pulmonary hypertension exhibit elevated CEC levels.
  • To determine the correlation between CEC counts and pulmonary artery pressures.
  • To characterize the phenotype of CECs in pulmonary hypertension patients.

Main Methods:

  • Isolated CECs from 14 pulmonary hypertension patients (5 PPH, 11 secondary pulmonary hypertension [SPH]) and 12 controls.

Related Experiment Videos

  • Compared CEC numbers between patient groups and controls.
  • Assessed correlation between CEC counts and hemodynamic parameters (systolic, diastolic, mean pulmonary artery pressures).
  • Analyzed CEC expression of CD36 and E-selectin markers.

    • Main Results:

    • CECs were significantly increased in both PPH and SPH patients compared to controls (33.1 ± 1.9 [PPH], 27.2 ± 6.9 [SPH] vs. 3.5 ± 1.3 [controls], p < 0.001).
    • CEC counts positively correlated with systolic, diastolic, and mean pulmonary artery pressures.
    • Approximately 50% of CECs expressed CD36 (microvascular origin marker), and 25% expressed E-selectin (activation marker).

    Conclusions:

    • Pulmonary hypertension patients show a marked increase in circulating endothelial cells.
    • CEC levels are associated with pulmonary artery pressure, indicating potential as a disease biomarker.
    • CECs may originate from the pulmonary vasculature, including plexiform lesions in plexogenic pulmonary hypertension.
    • Circulating endothelial cells offer a potential non-invasive method to study severe pulmonary hypertension pathobiology.