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Updated: Aug 4, 2026

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
Published on: June 23, 2016
[Connection of ethidium bromide with single-stranded DNA]
P O Vardevanian1, A P Antonian, M A Parsadanian
1Biological Department, Yerevan State University, ul. A. Manukyana 1, Yerevan, 375025 Armenia.
Abstract:
The interaction of ethidium bromide with single-stranded synthetic and natural polynucleotides at high temperatures (t = 70 degrees C) and low pH values (pH 3.0) was studied. The isotherms of adsorption of ethidium bromide on single-stranded DNA were obtained. Two modes of binding of single-stranded DNA, strong and weak, were revealed. The values of the corresponding constants of interaction of this ligand and the number of bases per one binding site were determined.
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