[The chimerism and GVT effects on murine mini-transplantation model]

We established a murine mini-transplantation model to examine chimerism and graft versus tumor effects(GVT) using with C57BL/6(donor) and(C57BL/6 x BALB/c) (CBF1) (recipient) combination and a IgE producing plasmacytoma cell clone (B53) of BALB/c origin. The chimerism and graft versus tumor effects(GVT) were observed. Two experimental groups were prepared. One is mini-transplantation group (G2): recipients irradiated with 200 rad were inoculated with B53 cells, along with donor cells, the other is bone marrow transplantation group(G4): recipients irradiated with 800 rad were inoculated with B53, along with donor cells. The full chimera was observed one week after transplantation in G4, but that was seen 3 week after transplantation. The GVT effects were observed in both groups. However, GVT effects on G4 is stronger than that on G2 in pathological findings and serum IgE level.