Related Experiment Video
Updated: Aug 30, 2026

Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
[The isolation and identification of a new alpha-pyrone from Opuntia dillenii]
Ying-kun Qiu1, De-qiang Dou, Yu-ping Pei
1Shenyang Pharmaceutical University, Shenyang 110016, China.
Aim:
To study the chemical composition of Opuntia dillenii Haw.
Methods:
Many kinds of chromatography methods were used to separate the chemical constituents. Their structures were determined by NMR and MS spectral analysis.
Results:
A new compound, together with five known compounds, were isolated from the 80% ethanolic extract of the stems.
Conclusion:
The new compound was identified as 4-ethoxyl-6-hydroxymethyl-alpha-pyrone. Compounds 1, 3, 4 and 5 were obtained for the first time from the genus of Opuntia, and they were: 3-O-methyl isorhamnein, 1-heptanecanol, vanillic acid, isorhamnetin-3-O-beta-D-rutinoside. Ruin was isolated from this plant for the first time.

