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Updated: Aug 30, 2026

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers
Published on: November 22, 2015
An improved method for the capillary gas chromatographic derivatization of polyhydroxylated steroids having
Takashi Iida1, Keisuke Shiraishi, Shoujiro Ogawa
1Department of Chemistry, College of Humanities & Sciences, Nihon University, Setagaya, Sakurajousui, Tokyo 156-8550, Japan. takaiida@chs.nihon-u.ac.jp
Abstract:
An improved method for a suitable derivatization of polyhydroxylated steroids having one or two tert-hydroxyl groups at the 5beta-, 14alpha-, 17alpha-, 24-, and/or 25-positions by capillary gas chromatography (CGC) is described. By using trimethylsilyl triflate as a silylating reagent and 2,6-lutidine as a catalyst, each of 5beta-cholane and 5alpha-cholestane series of steroids was successfully transformed into trimethylsilyl (TMS) ether derivatives to give a single CGC peak under mild conditions. More bulky triethylsilyl (TES) etherification of 14alpha- and 17alpha-hydroxy compounds provided multiple CGC peaks arising from completely- and/or incompletely-derivatized TES ethers accompanied by their thermal elimination products.
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