Microwave cell death: Immunohistochemical and enzyme histochemical evaluation

In Japan, microwave coagulation therapy (MCT) has been used for the management of primary and metastatic liver cancer. Needle biopsy examination from the lesion has frequently shown the presence of nucleated cancer cells in histopathological examinations, prompting the conclusion that cancer cells are not completely eliminated by microwave therapy, whereas computed tomography and ultrasonography examinations show tumor regression. To determine whether microwave-treated tissue contains functionally viable cells, an examination of the Na+-K+-ATPase protein and its activity using immunohistochemical and enzyme histochemical methods were carried out in microwave-treated rat liver. Four concentric, morphologically identifiable zones around the microwave probe needle appeared 2 days after treatment. Zone A, which was between the innermost spongy zone and the outer necrotic zone, contained only slight morphological alterations in the hepatocytes, which had slightly hyperchromatic nuclei and mildly eosinophilic cytoplasm. The hepatocytes in zone A were found to be positive for the Na+-K+-ATPase antigenicity but negative for enzyme activity, indicating that zone A was undergoing cell death, although morphologically this was not discernible. This type of cell death caused by microwave treatment is morphologically different from previously known types of cell death, either oncosis or apoptosis.