Related Experiment Video
Updated: Aug 14, 2026

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
Published on: January 29, 2010
Chagas' disease diagnosis: comparative analysis of recombinant ELISA with conventional ELISA and the
A A M Gadelha1, A F A Verçosa, V M B Lorena
1Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brazil.
Background And Objectives:
Serological screening for Chagas' disease in the blood banks of South America is carried out by using two different assays that generally show a high number of inconclusive results. To establish a combination of two tests that can minimize the number of inconclusive results, we compared a recombinant enzyme-linked immunosorbent assay (ELISA) with two conventional tests.
Materials And Methods:
Serum samples from chagasic patients (n = 112), from non-chagasic individuals (n = 143) and from patients with other diseases (n = 32) were tested using three assays: recombinant ELISA (Rec-ELISA); conventional ELISA (Con-ELISA); and the indirect haemagglutination (IHA) test.
Results:
When we evaluated the data by matching the Rec-ELISA and the IHA test, 52 inconclusive results were obtained. When Rec-ELISA and Con-ELISA were matched, only four inconclusive results were observed.
Conclusions:
Our investigation indicates that the use of two ELISAs with different antigen preparations provides an effective test combination for blood bank screening of Chagas' disease.
Related Concept Videos
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
American Trypanosomiasis

