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Characterization of a novel single-stranded RNA mycovirus in Pleurotus ostreatus

Hyun Jae Yu1, Dongbin Lim, Hyun-Sook Lee

  • 1Department of Microbiology and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Korea.

Virology
|October 1, 2003
PubMed

Insights

A novel mycovirus, oyster mushroom spherical virus (OMSV), was identified in diseased oyster mushrooms. This spherical virus has a single-stranded RNA genome and shares similarities with plant tymoviruses.

Area of Science:

  • Mycology
  • Virology
  • Plant Pathology

Background:

  • Oyster mushrooms are susceptible to severe die-back disease.
  • Mycoviruses can impact fungal health and productivity.
  • Understanding viral pathogens is crucial for disease management in cultivated fungi.

Purpose of the Study:

  • To isolate and characterize a mycovirus associated with oyster mushroom die-back disease.
  • To determine the genomic structure and potential functions of the identified mycovirus.
  • To investigate the phylogenetic relationship of the mycovirus with known viral groups.

Main Methods:

  • Isolation of the mycovirus from infected oyster mushrooms.
  • Electron microscopy for determining virus morphology.
  • RNA extraction, sequencing, and bioinformatic analysis to determine genomic features.
  • Sequence comparison with known viral genomes.

Main Results:

  • A 27-nm spherical mycovirus, named oyster mushroom spherical virus (OMSV), was isolated.
  • OMSV possesses a positive-sense single-stranded RNA genome (5.784 kb) with seven open reading frames (ORFs).
  • ORF1 contains RNA-dependent RNA polymerase (RdRp) and helicase motifs; ORF2 encodes the coat protein. Genomic organization resembles tymoviruses.

Conclusions:

  • OMSV is a distinct mycovirus implicated in oyster mushroom die-back disease.
  • The genomic characteristics of OMSV suggest a relationship with the tymovirus family.
  • Further research is needed to understand OMSV's replication cycle and pathogenicity in oyster mushrooms.

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