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Related Experiment Videos

Clathrin self-assembly involves coordinated weak interactions favorable for cellular regulation.

Diane E Wakeham1, Chih-Ying Chen, Barrie Greene

  • 1The G. W. Hooper Foundation, Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0552, USA.

The EMBO Journal
|October 1, 2003
PubMed
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Clathrin assembly into polyhedral coats relies on coordinated weak interactions between leg segments, driven by trimerization. This process allows cellular regulation of vesicle formation for endocytosis.

Area of Science:

  • Cell Biology
  • Structural Biology
  • Biochemistry

Background:

  • Clathrin self-assembles into polyhedral coats, essential for receptor sorting in the endocytic pathway.
  • A clathrin triskelion is formed by three heavy chains with distinct leg segments and terminal domains.

Purpose of the Study:

  • To define the contributions of proximal and distal leg segment interactions to clathrin assembly.
  • To measure the interaction strength between clathrin leg segments.

Main Methods:

  • Investigated self-assembly of chimeric clathrin constructs with modified trimerization domains.
  • Measured the affinity of proximal and distal leg segments for homo- and heterodimer formation.

Main Results:

  • Individual proximal and distal leg segments showed insufficient affinity for stable dimerization.

Related Experiment Videos

  • Chimeric constructs with replaced trimerization domains exhibited reversible self-assembly.
  • Clathrin assembly is driven by the coordination of weak leg segment affinities through trimerization.

    • Conclusions:

    • Clathrin polymerization depends on multiple weak interactions, similar to other biopolymers.
    • The assembly process is sensitive to environmental changes, enabling cellular regulation of vesicle formation.