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Related Experiment Videos

Behavioral study on the gracile axonal dystrophy (GAD) mutant mouse.

K Yamazaki1, T Nakazawa, M Matsunaga

  • 1Tsukuba Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan.

Jikken Dobutsu. Experimental Animals
|October 1, 1992
PubMed
Summary

Gracile axonal dystrophy (GAD) mouse models show early motor and sensory deficits. The rotarod test effectively detects motor incoordination and paresis progression in GAD mice.

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Area of Science:

  • Neuroscience
  • Genetics
  • Animal Models

Background:

  • Gracile axonal dystrophy (GAD) is a genetic disorder affecting motor function and sensation.
  • GAD mice exhibit a progressive shift in symptoms from sensory ataxia to paresis.

Purpose of the Study:

  • To investigate motor function and pain sensation in GAD mutant mice.
  • To evaluate the utility of the tail-flick and rotarod tests in characterizing GAD progression.

Main Methods:

  • Utilized GAD (gad/gad) and normal sibling mice (gad/+ or +/+) aged 5-11 weeks.
  • Assessed pain sensation using the tail-flick test.
  • Evaluated motor coordination with the rotarod test.

Main Results:

  • Female GAD mice showed reduced pain sensitivity (shorter latency) at 6 and 8 weeks.

Related Experiment Videos

  • Rotarod testing revealed significant motor incoordination in GAD mice starting at 5 weeks (males) and 6 weeks (females).
  • GAD mice experienced a sharp decline in rotarod performance after 9 weeks, indicating paresis progression.

    • Conclusions:

    • The tail-flick test may indicate early nociceptive pathway dysfunction in GAD.
    • The rotarod test is a sensitive method for quantifying motor deficits and tracking GAD progression.
    • Rotarod testing can aid in early GAD mouse identification and drug efficacy evaluation for ataxia and paresis.