Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-speed chiral separations on a microchip with UV detection.

Martin Ludwig1, Frank Kohler, Detlev Belder

  • 1Abteilung für Chromatographie, Max-Planck-Institut für Kohlenforschung, Mülheim an der Ruhr, Germany.

Electrophoresis
|October 1, 2003
PubMed
Summary

This study introduces rapid chiral separations using microchip electrophoresis (MCE) on quartz chips, eliminating the need for fluorescence tagging. This method achieves high-throughput screening for enantiomeric excess determination in under a minute.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Employing exon capture to resolve species-level relationships in Australian Arid Zone camaenid land snails.

Molecular phylogenetics and evolution·2026
Same author

Development of an ultra-fast polarity-switching ion mobility spectrometer ready for coupling with supercritical fluid chromatography.

Journal of chromatography. A·2025
Same author

A Conceptual Framework for the Crystallizability of Organic Compounds.

Journal of the American Chemical Society·2025
Same author

Phylogeny of freshwater mollusc genus Brotia H. Adams, 1866 (Gastropoda: Pachychilidae) from north-east India.

Invertebrate systematics·2025
Same author

Fast Chemical Analysis of Droplets Unlocked by Ultra-Fast Ion Mobility Spectrometry.

Analytical chemistry·2025
Same author

A Microfluidic Device to Realize Electrochemically Controlled SERS Detection in HPLC.

Analytical chemistry·2025

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Microfluidics

Background:

  • Chiral separations are crucial in pharmaceuticals and chemical analysis.
  • Traditional methods often require complex sample preparation, such as fluorescence tagging.
  • Microchip electrophoresis (MCE) offers potential for faster and more efficient separations.

Purpose of the Study:

  • To develop a rapid chiral separation technique using microchip electrophoresis.
  • To demonstrate the omission of fluorescence tagging for detection.
  • To assess the capability of MCE for high-throughput screening and enantiomeric excess determination.

Main Methods:

  • Utilized a microchip electrophoresis instrument with a linear imaging UV-detector.
  • Employed sulfated cyclodextrins as chiral selectors on microfluidic quartz chips.

Related Experiment Videos

  • Separated a variety of basic and acidic chiral compounds.

    • Main Results:

    • Achieved baseline separation of 19 compounds in less than 1 minute with high reproducibility (RSD < 7%).
    • Reported the fastest enantiomer separation to date at 2.5 seconds.
    • Successfully determined high enantiomeric excess (ee) values, detecting 2% of a minor enantiomer.
    • Separated a mixture of 3 chiral drugs in under 11 seconds using a 12 mm separation length.

    Conclusions:

    • Microchip electrophoresis is a powerful tool for rapid chiral analysis.
    • The developed method eliminates the need for cumbersome fluorescence tagging.
    • MCE shows significant potential for high-throughput screening applications in chiral analysis.