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Related Experiment Videos

Microbial quantification in activated sludge: the hits and misses.

S J Hall1, J Keller, L L Blackall

  • 1School of Molecular and Microbial Sciences, The University of Queensland, St. Lucia 4072, Australia. s.hall1@uq.edu.au

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|October 2, 2003
PubMed
Summary

Monitoring biological nutrient removal (BNR) processes requires quantifying key microorganisms. This study evaluated real-time PCR and FISH for quantifying Nitrospira, crucial for nitrate production, to improve wastewater treatment performance.

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Area of Science:

  • Environmental Microbiology
  • Wastewater Treatment Technologies
  • Molecular Biology

Background:

  • Traditional wastewater treatment monitoring relies on physical and chemical parameters.
  • Biological Nutrient Removal (BNR) processes require monitoring of key microbial populations for optimal performance.
  • In situ identification and rapid quantification of microorganisms are essential for effective BNR process control.

Purpose of the Study:

  • To explore and evaluate molecular genetic techniques for quantifying Nitrospira, a key microorganism in nitrification within BNR systems.
  • To compare the efficacy of real-time polymerase chain reaction (PCR) and fluorescence in situ hybridisation (FISH) for Nitrospira quantification.
  • To investigate the correlation between Nitrospira abundance and nitrate production rates in BNR.

Main Methods:

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  • Real-time polymerase chain reaction (PCR) was employed for microbial quantification.
  • Fluorescence in situ hybridisation (FISH) coupled with digital image analysis was utilized.
  • Batch tests were conducted to determine nitrate production rates.

Main Results:

  • Both real-time PCR and FISH were assessed for their ability to quantify Nitrospira populations.
  • The study attempted to correlate quantitative microbial data with observed nitrate production rates.
  • Advantages and disadvantages of each molecular quantification method were discussed.

Conclusions:

  • Accurate quantification of key microorganisms like Nitrospira is vital for optimizing BNR processes.
  • Molecular techniques such as real-time PCR and FISH offer promising avenues for in situ monitoring of BNR systems.
  • Further development of these methods can lead to improved wastewater treatment efficiency and performance.