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Related Concept Videos

NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range. Consider...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.

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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
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Adiabatic single scan two-dimensional NMR spectrocopy.

Philippe Pelupessy1

  • 1Contribution from the Département de Chimie, associé au CNRS, Ecole Normale Supérieure, 24 rue Lhomond, 75231 Paris Cedex 05, France. philippe.pelupessy@ens.fr

Journal of the American Chemical Society
|October 2, 2003
PubMed
Summary

New adiabatic pulse schemes enhance single scan 2D Nuclear Magnetic Resonance (NMR) experiments. These methods offer advantages and are applicable to both homo- and heteronuclear studies.

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Area of Science:

  • Chemistry
  • Spectroscopy
  • Nuclear Magnetic Resonance (NMR)

Background:

  • Two-dimensional NMR (2D NMR) experiments are crucial for molecular structure elucidation.
  • Traditional 2D NMR methods often require long acquisition times, limiting their applicability.
  • Developing faster NMR techniques is essential for efficient structural analysis.

Purpose of the Study:

  • To introduce novel excitation schemes for rapid 2D NMR.
  • To demonstrate the advantages of these new schemes.
  • To present applications in various NMR experiments.

Main Methods:

  • Utilizing adiabatic pulses for excitation.
  • Implementing single scan acquisition protocols.
  • Developing new pulse sequences for 2D NMR.

Main Results:

  • Successful implementation of adiabatic pulse-based excitation schemes.
  • Demonstration of enhanced performance in single scan 2D NMR.
  • Presentation of successful applications in both homonuclear and heteronuclear NMR.

Conclusions:

  • Adiabatic pulse schemes provide an effective approach for accelerating 2D NMR.
  • These methods offer significant advantages for rapid data acquisition.
  • The introduced techniques are versatile and applicable to a broad range of NMR studies.