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Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
08:18

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Published on: December 31, 2014

An integrated database of genes responsive to the Myc oncogenic transcription factor: identification of direct

Karen I Zeller1, Anil G Jegga, Bruce J Aronow

  • 1Division of Hematology, Department of Medicine, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Genome Biology
|October 2, 2003
PubMed

Insights

We created a Myc Target Gene database to identify genes regulated by the Myc oncogenic transcription factor. This resource aids in understanding cancer-causing genetic networks.

Area of Science:

  • Oncology
  • Genetics
  • Bioinformatics

Background:

  • The Myc oncogenic transcription factor plays a crucial role in cell growth and cancer development.
  • Understanding Myc's regulatory network is essential for deciphering cancer genesis.

Purpose of the Study:

  • To develop a comprehensive database of Myc-responsive genes.
  • To facilitate the identification and functional grouping of Myc target genes.
  • To aid in the understanding of genetic regulatory networks in cancer.

Main Methods:

  • Creation of the Myc Target Gene database, prioritizing genes by experimental evidence.
  • Clustering of responsive genes into functional groups.
  • Phylogenetic sequence comparisons to predict c-Myc binding sites.
  • Validation of predicted binding sites using chromatin immunoprecipitation assays.

Main Results:

  • A curated database of genes responsive to the Myc transcription factor.
  • Prioritized candidate target genes with functional annotations.
  • Validated c-Myc binding sites through experimental assays.

Conclusions:

  • The Myc Target Gene database is a valuable resource for cancer research.
  • This database enhances the understanding of Myc-driven genetic regulatory networks.
  • It provides a foundation for further investigation into oncogenesis.

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