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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
Published on: July 3, 2018
Reconstitution and characterization of NtrC protein in a deep-sea piezophilic bacterium, Shewanella violacea strain
Hiroaki Kawano1, Akihiko Ikegami, Kaoru Nakasone
1Department of Applied Chemistry, Faculty of Engineering, Toyo University, Kawagoe, Saitama, Japan.
Bioscience, Biotechnology, and Biochemistry
|October 2, 2003
Abstract:
NtrC protein of piezophilic Shewanella violacea was overexpressed and purified, to confirm the protein-DNA interaction. An electrophoretic mobility shift assay demonstrated that the NtrC recognizes the sequence for NtrC binding within the region upstream of the glnA operon. Western blot analysis also showed that the NtrC is expressed at a higher level under high-pressure conditions than under atmospheric pressure conditions.

