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Construction of a homologous selectable marker gene for Lentinula edodes transformation
Toshikazu Irie1, Toshitsugu Sato, Kumiko Saito
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
Bioscience, Biotechnology, and Biochemistry
|October 2, 2003
Abstract:
We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced a point mutation that confers carboxin resistance into it. The mutant gene successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased the transformation efficiency by about two-fold.
